Carboxyl-terminal protein sequencing method and kit

Details Carboxyl-terminal protein sequencing method and kit Carboxyl-terminal protein sequencing method and kit

1990-06-21

1991-11-12

Hill, Jr., Robert J.

Chemistry: analytical and immunological testing

Peptide, protein or amino acid

Amino acid or sequencing procedure

422 61, 436 98, 436111, 436120, 436128, 530345, 530402, 530408, 530409, 530410, G01N 3368

Patent

active

050647679

ABSTRACT:
Polypeptides are sequenced from the carboxyl terminus by treating the peptide with an iodoxybenzene, followed by removal of the excess reagent and reaction with 2-aminothiophenol at an elevated temperature. The resulting benzthiazolidine is then analyzed to determine the C-terminal amino acid which has been cleaved. A kit which contains the iodoxybenzene and 2-aminothiophenol reagents is separate containers is also disclosed.

REFERENCES:
Parham et al., Biochem. Biophys. Res. Comm., vol. 80, No. 1, pp. 1-6, 1978.
Loudon et al., Tetrahedron Letters, No. 5, pp. 437-440, 1978.

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