Carbonyl reductase, gene thereof and method of using the same

Details Carbonyl reductase, gene thereof and method of using the same Carbonyl reductase, gene thereof and method of using the same

2007-05-22

2007-05-22

Prouty, Rebecca E. (Department: 1652)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Oxidoreductase

C435S220000, C435S156000, C435S069100, C435S252200, C536S023200

Reexamination Certificate

active

10528000

ABSTRACT:
The present invention provides a novel polypeptide efficiently forming (R)-N-benzyl-3-pyrrolidinol, a polynucleotide coding for said polypeptide, and use of the same.The present invention relates to a polypeptide having the following physical and chemical properties (1) to (4):(1) activity: acting on N-benzyl-3-pyrrolidinone with NADH or NADPH as a coenzyme, to form (R)-N-benzyl-3-pyrrolidinol;(2) optimum pH for activity: 5.5 to 6.0;(3) optimum temperature for activity: 50° C. to 55° C.;(4) molecular weight: about 55,000 as determined by gel filtration analysis, about 28,000 as determined by SDS polyacrylamide gel electrophoresis analysis.The present invention also relates to a polypeptide comprising the amino acid sequence shown under SEQ ID NO:1 in the sequence listing, a polynucleotide coding for said polypeptide, and a transformant producing said polypeptide at high levels.

REFERENCES:
patent: 2004/0235124 (2004-11-01), Kawano et al.
patent: 4-218384 (1992-08-01), None
patent: 6-141876 (1994-05-01), None
patent: 654534 (1995-05-01), None
patent: 8-89261 (1996-04-01), None
patent: 10-150997 (1998-06-01), None
patent: 11-215995 (1999-08-01), None
patent: 03-31636 (2003-04-01), None
Li, Zhi, et al., “Preparation of optically activeN-benzyl-3-hydroxypyrrolidine by enzymatic hydroxylation,”Tetrahedron: Asymmetry, 10 (1999) 1323-1333.

Affiliated with

Also associated with

Rating

Carbonyl reductase, gene thereof and method of using the same does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Carbonyl reductase, gene thereof and method of using the same, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Carbonyl reductase, gene thereof and method of using the same will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3744871