Carbonyl reductase, gene thereof and method of using the same

Details Carbonyl reductase, gene thereof and method of using the same Carbonyl reductase, gene thereof and method of using the same

2006-04-25

2006-04-25

Rao, Manjunath (Department: 1652)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Oxidoreductase

C435S004000, C435S006120, C435S069100, C435S071100, C435S252300, C435S320100, C435S252330, C435S155000, C536S023200, C536S023700

Reexamination Certificate

active

07033808

ABSTRACT:
The present invention provides a novel polypeptide producing (S)-N-benzyl-3-pyrrolidinol, a DNA coding for it and a method of using them.A polypeptide having the following physicochemical properties (1) to (5):(1) Action: It asymmetrically reduces N-benzyl-3-pyrrolidinone to produce (S)-N-benzyl-3-pyrrolidinol with NADPH as a coenzyme;(2) Optimum action pH: 4.5 to 5.5;(3) Optimum action temperature: 40° C. to 45° C.;(4) Molecular weight: About 29,000 as determined by gel filtration analysis, about 35,000 as determined by SDS-polyacrylamide gel electrophoresis analysis;(5) Inhibitor: It is inhibited by the divalent copper ion.Further, a polypeptide having the amino acid sequence shown under SEQ ID NO:1 in the sequence listing; ora polypeptide having an amino acid sequence obtainable from the amino acid sequence shown under SEQ ID NO:1 in the sequence listing by substitution, insertion, deletion and/or addition of one or more amino acids andhaving enzyme activity in asymmetrically reducing N-benzyl-3-pyrrolidinone to produce (S)-N-benzyl-3-pyrrolidinol.

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Database Unit Prot Online, “OXIDOREDUCTASE”, EBI Accession No. UNIPROT:Q67RQ0 (XP002311041), Oct. 25, 2004.

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