Carbonyl reductase, gene therefor and use thereof

Details Carbonyl reductase, gene therefor and use thereof Carbonyl reductase, gene therefor and use thereof

2011-08-30

2011-08-30

Carlson, Karen Cochrane (Department: 1652)

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

C435S189000, C435S069100, C435S128000, C558S410000

Reexamination Certificate

active

08008461

ABSTRACT:
The present invention is to provide a process for efficiently producing an optically active alcohol including (R)-3-hydroxy-3-phenylpropanenitrile. One of the features of the present invention is a polypeptide having an activity of asymmetrically reducing 3-oxo-3-phenylpropanenitrile isolated from a microorganism belonging to the genusCandidato produce (R)-3-hydroxy-3-phenylpropanenitrile, DNA encoding the polypeptide and a transformant of producing the polypeptide. Another feature of the present invention is a process for producing an optically active alcohol such as (R)-3-hydroxy-3-phenylpropanenitrile by reducing a carbonyl compound such as 3-oxo-3-phenylpropanenitrile by use of the polypeptide or the transformant.

REFERENCES:
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Current Protocols in Molecular Biology, Hybridization Analysis of DNA Blots, Chapter 2.10, 1993.
Alignment of Seq ID No. 2 with Seq ID No. 1 of Kizaki et al. (US 6,645,746), http://blast.ncbi.nlm.nih.gov/Blast.cgi, performed on NCBI Blast web site Mar. 6, 2009.
Result 1, search in issued patents polynucleotide database, alignment of instant Seq ID No. 1 with Kizaki et al., US 6,645,746 B1, Seq ID No. 2, performed on May 7, 2009.
H. Guo et al., “Protein Tolerance to Random Amino Acid Change”, PNAS 101(25):9205-9210, 2004.
Score search results 2-6, search in Geneseq protein database, alignment of instant Seq ID No. 2 with a sequence of each cited reference, performed on Mar. 3, 2009, 7 pages.
Y. Yasohara et al., “Stereoselective reduction of alkyl 3-oxobutanoate by carbonyl reductase fromCandida magnoliae”,Tetrahedron: Asymmetry, 2001, p. 1713-1718, vol. 12.
J.R. Dehli et al., “Enantio- and chemoselective bioreduction of β-keto nitriles by the fungusCurivularia lunata”, Tetrahedron: Asymmetry, 2000, p. 3693-3700, vol. 11.

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