Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1985-07-09
1988-10-25
Warden, Robert J.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 7, 536 26, 536 27, 536 28, 935 78, 935 77, 436501, 548136, 548303, 549223, C12Q 168
Patent
active
047804050
ABSTRACT:
Nucleic acid hybridization probes are provided which comprise nucleoside bases or terminal nucleotide phosphates chemically linked to aromatic sulfonamide inhibitors of carbonic anhydrase. Methods of preparing probes of the invention, intermediates used in such methods, and methods of using the probes of the invention in hybridization assays are also provided. A probe of the invention is detected by binding to it a reporter group, such as a homopolymer or heteropolymer of enzymes, which includes a carbonic anhydrase which binds to the inhibitor linked to the probe, and then detecting the bound reporter group, as by production of a fluorescent or colored product in a reaction catalyzed by an enzyme component of the reporter group. Also provided are enzyme immunoassays wherein detection of antibody is by a process which comprises a chemical reaction catalyzed by a carbonic anhydrase.
REFERENCES:
patent: 4134792 (1979-01-01), Boguslaski et al.
Draper et al. "A Method for Linking Fluorescent Labels to Polynucleotides Application to Studies of Ribosome-Ribonucleic Acid Interactions," Biochemistry, 1980,19,1774-1781.
Draper, "Attachment of Reporter Groups to Specific Selected Cytidine Residues in RNA using a Bisulfite-Catalyzed Transamination Reaction," Nucleic Acids Research, 1987, 12, 989-1002.
Mumford et al. "Purification of a membrane-bound metalls-endopeptidase from porcine kidney that degrades peptide hormones," PNAS, 78, Nall pp. 6623-6627, Nov. 1981.
Nitta et al., "A New Reaction Useful for Chemical Cross-Linking Between Nucleic Acids and Proteins," FEBS Letters 166, 194-198 (1984).
Negishi et al., "N.sup.4 -Aminocytidine: Formation, Reactivity, and Mutagenicity," Nucl. Acids Res., Symp. Ser. No. 12, 29-30 (1983).
Negishi et al. "N.sup.4 -Aminocytidine, A nucleoside Analog that Has an Exceptionally High Mutagenic Activity," Nucl. Acids Res. 11, 5223-5233 (1983).
Smith, "Hydrazine Derivatives," The Chemistry of Open-Chain Organic Nitrogen Compounds, vol. II, pp. 119-209. W. A. Benjamin, N.Y., N.Y. (1966).
Leary, "A Staining Procedure for Cattle and Bison Carbonic Anhydrase Using Fluorescein Diacetate," Anim. Blood. Grps. Biochem. Genet. 9, 65-67 (1978).
Epton et al., "Water-Soluble Coloured Covalent Conjugates of Carbonic Anhydrase and N-(sym-Trinitroaryl)polyacrylamide/Acrylhydrazide Co-Polymers," Biochem. Soc. Trans. 5, 277-279 )1977).
Livesey, "On the Colorimetric Method of Assaying Carbonic Anhydrase (EC 4.2.1.1)," Anal. Biochem. 77, 552-561 (1977).
Whitney et al., "Inhibition of Human Erythrocyte Carbonic Anhydrase B by Chloroacetyl Sulfonamides with Labeling of the Active Site," J. Biol. Chem. 242, 4206-4211 (1967).
Langer et al., "Enzymatic Synthesis of Biotin-labeled Polynucleotides Novel Nucleic Acid Affinity Probes," Proc. Natl. Acad. Sci. (U.S.A.) 78, 6633-6637 (1981).
Chu and Orgel, "Detection of Specific DNA Sequences with Short Biotin-labeled Proves," DNA 4, 327-331 (1985).
Chu and Orgel, "Nonenzymatic Sequence-specific Cleavage of Single-stranded DNA," Proc. Natl. Acad. Sci. (U.S.A.) 82, 963-976 (1985).
Chu et al., "Derivatization of Unprotected Polynucleotides," Nucl. Acids Res. 11, 6513-6529 (1983).
Hayatsu, "Bisulfite Modification of Nucleic Acids and their Constitutents," Pro. in Nucl. Acid Res. and Mol. Biol. 16, 75-124 (1976).
Ghosh Soumitra
Kaiser Emil T.
Musso Gary F.
Orgel Leslie E.
Wahl Geoffrey M.
Siska Diagnostics Inc.
Spiegel Carol A.
Warden Robert J.
LandOfFree
Carbonic anhydrase inhibitor-tagged nucleic acid probes does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Carbonic anhydrase inhibitor-tagged nucleic acid probes, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Carbonic anhydrase inhibitor-tagged nucleic acid probes will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2269263