Chemistry: molecular biology and microbiology – Plant cell or cell line – per se ; composition thereof;... – Culture – maintenance – or preservation techniques – per se
Patent
1998-05-12
2000-11-07
Robinson, Douglas W.
Chemistry: molecular biology and microbiology
Plant cell or cell line, per se ; composition thereof;...
Culture, maintenance, or preservation techniques, per se
435419, 435468, 4353201, 536 236, 536 241, 800295, 800298, C12N 1529, C12N 1582, A01H 400, A01H 500
Patent
active
061435627
ABSTRACT:
A carbon-based process for the selection of heterotrophically cultured plant cells is contemplated as are plants transformed using that process and a kit useful for effecting such a transformation. Plant cells are transformed with a heterologous DNA segment that contains two expression cassettes. The first cassette contains a gene that encodes a heterologous enzyme that on expression converts a growth-limiting (encrypted) carbon source that does not support growth and proliferation of non-transformed plant cells into a carbon source that supports growth and proliferation of transformed plant cells. The second cassette contains a second gene to be expressed in the transformed plant cells. A mixture transformed and non-transformed plant cells is cultured under heterotrophic culture conditions with the growth-limiting carbon source as the only carbon source. Inasmuch as only the transformed cells express the heterologous enzyme that converts the carbon source present into a carbon source that is useful to support growth and proliferation, only transformed cells grow and proliferate, and those cells are selected.
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Stalker et al., "Herbicide Resist
Braun, III Carl Joseph
Green Charles Edward
Trulson Anna Julia
Haas Thomas
Robinson Douglas W.
Seminis Vegetable Seeds
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