Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Fungi
Patent
1986-07-23
1989-08-15
Weimar, Elizabeth C.
Chemistry: molecular biology and microbiology
Micro-organism, per se ; compositions thereof; proces of...
Fungi
435938, 4351722, 4351723, 43524026, 935 66, 935 69, 935 97, C12N 116, C12N 1500, C12N 500
Patent
active
048574678
ABSTRACT:
Yeast strains are transformed with, and selected for, using DNA fragments encoding a gene function not present in the wild-type yeast strains. The invention requires transforming the yeast with DNA fragments which comprise the gene function which the wild-type yeast strain is lacking; regeneration of the transformants on a non-selective regeneration medium; then selection of those colonies which are capable of growth on a carbon and energy source which requires expression of the gene function provided by the DNA fragment. Thus, transformation with such DNA fragments allows for positive selection of transformed strains.
REFERENCES:
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Fuke Motohiro
Sreekrishna Kotikanyadan
Carson P.
Phillips J. E.
Phillips Petroleum Company
Weimar Elizabeth C.
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