Carbomycin resistance-conferring gene, designated carb, for use

Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses

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435 691, 435 34, 435 76, 435 91, 4351721, 4352535, 435320, 435886, 530350, 536 27, C12N 1500, C12N 120, C12N 100, C12P 2100, C12P 2102, C12P 1962, C12P 1934, C12R 1465, C07K 1300, C07H 1512

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049140302

ABSTRACT:
The carB gene is a novel carbomycin resistance-conferring gene isolated from Streptomyces thermotolerans and used to construct a number of cloning vectors for use in Streptomyces and related organisms. One such coloning vector, plasmid pOJ159, can be obtained in S. griseofuscus C581 under the accession number NRRL 18090. S. lividans and S. griseofuscus are the preferred hosts when the carB gene is used to select carbomycin-resistant Streptomyces transformants.

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