Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage
Patent
1995-03-31
1997-02-25
Springer, David B.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving virus or bacteriophage
435 71, 548546, C12Q 168, C12Q 170, G01N 3353
Patent
active
056057911
ABSTRACT:
The invention provides heterobifunctional cross-linking reagents and methods of using the cross-linking reagents. The cross-linking reagents of the invention combine a nucleophilic hydrazide residue with an electrophilic maleimide residue, allowing coupling of aldehydes to free thiols. In the methods of the invention, human immunodeficiency virus (HIV) infected cells can be detected using conjugates that include CD4 molecules conjugated to detectable markers via the disclosed cross-linking reagents.
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Ashkenazi Avi J.
Chamow Steven M.
Kogan Timothy P.
Dreger Ginger R.
Genentech Inc.
Marschang Diane L.
Springer David B.
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