Capturing sequences adjacent to Type-IIs restriction sites for g

Details Capturing sequences adjacent to Type-IIs restriction sites for g Capturing sequences adjacent to Type-IIs restriction sites for g

1995-06-07

1998-01-20

Chambers, Jasemine C.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

4351723, C12Q 168, C12N 1509, C12N 1510

Patent

active

057100004

ABSTRACT:
The present invention relates to novel methods for sequencing and mapping genetic markers in polynucleotide sequences using Type-IIs restriction endonucleases. The methods herein described result in the "capturing" and determination of specific oligonucleotide sequences located adjacent to Type-IIs restriction sites. The resulting sequences are useful as effective markers for use in genetic mapping, screening and manipulation.

REFERENCES:
patent: 5002867 (1991-03-01), Macevicz
patent: 5143854 (1992-09-01), Pirrung
patent: 5202231 (1993-04-01), Drmanac
patent: 5508169 (1996-04-01), Deugau et al.
Brenner et al., "DNA fingerprinting by sampled sequencing," Proc. Natl. Acad. Sci. USA 86:8902-8906 (1989).
Drmanac, R. "Genome Sequencing Machine," p. 60 (publication journal and date unknown).
Hoheisel, "Application of hybridization techniques to genome mapping and sequencing," Trends in Genetics 10(3):79-83 (1994).
Sapolsky et al., "Mapping genomic library clones using oligonucleotide arrays," Hilton Head Conf., SC, Sep. 17-21, 1994.
Smith, "Ligation-mediated PCR of restriction fragments from large DNA molecules," PCR Methods and Applications 2:21-27 (Cold Spring Harbor Laboratory Press, 1992).
Szybalski, "Universal restriction endonucleases: designing novel cleavage specificities by combining adapter oligodeoxynucleotide and enzyme moieties," Gene 40:169-173 (1985).
Szybalski et al., "Class IIS restriction enzymes--a review", Gene 100: 13-26, 1991.
Unrau et al., "Non-cloning amplification of specific DNA fragments from whole genomic DNA digests using DNA `indexers`", Gene 145: 163-169, Aug. 1994.

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