Capturing sequences adjacent to type-IIs restriction sites...

Details Capturing sequences adjacent to type-IIs restriction sites... Capturing sequences adjacent to type-IIs restriction sites...

2011-07-26

2011-07-26

Ketter, James S (Department: 1636)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S091200

Reexamination Certificate

active

07985547

ABSTRACT:
The present invention relates to novel methods for sequencing and mapping genetic markers in polynucleotide sequences using Type-IIs restriction endonucleases. The methods herein described result in the “capturing” and determination of specific oligonucleotide sequences located adjacent to Type-IIs restriction sites. The resulting sequences are useful as effective markers for use in genetic mapping, screening and manipulation.

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