Capsule chemistry analytical methods employing dioxetane chemilu

Details Capsule chemistry analytical methods employing dioxetane chemilu Capsule chemistry analytical methods employing dioxetane chemilu

1995-07-31

1997-05-20

Soderquist, Arlen

Chemistry: analytical and immunological testing

Automated chemical analysis

With a continuously flowing sample or carrier stream

436 63, 436166, 436172, 436501, G01N 3508, G01N 2176

Patent

active

056311672

ABSTRACT:
Improved assay methods utilizing stable, enzymatically triggered chemiluminescent 1,2-dioxetanes with improved water solubility are provided. Assays are performed by a capsule chemistry analytical assay method wherein fluid capsules comprising discrete aqueous segments containing either a chemiluminescent dioxetane reagent or an activating agent separated by an oil-based isolating fluid are flowed through a conduit, and subsequently reacted to produce light.
The improvement comprises using a dioxetane substituted with two or more water-solubilizing groups disposed on the dioxetane structure to provide the chemiluminescence. Compositions comprising such a dioxetane, a non-polymeric surfactant enhancer and optionally a fluorescer, provide enhanced chemiluminescence and eliminate the problem of reagent carryover when used in assays performed on capsule chemistry analytical systems.

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