Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Patent
1995-06-07
2000-08-29
Zitomer, Stephanie W.
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
435194, 536 231, 536 2431, 536 2432, 536 2531, 536 2534, C07H 2100, C07H 2102, C12N 912
Patent
active
061110957
ABSTRACT:
A method is provided for making synthetic capped RNAs. These compounds serve as substrates for the virally encoded endonuclease associated with influenza virus. We are able to assay for this unique and specific viral activity of cleavage of a capped RNA in vitro. Therefore, screening of inhibitors of this activity is possible. In addition, short non-extendible (due to their length or because of the modification of the 3'-end of the oligo, i.e. 3'-dA) RNAs are potent inhibitors of the cleavage of capped RNAs by influenza endonuclease. Finally, these compounds may be used to investigate viral and cellular mechanisms of transcription/translation or mRNA maturation.
REFERENCES:
patent: 4415732 (1983-11-01), Caruthers et al.
patent: 4725677 (1988-02-01), Koster et al.
patent: 5212295 (1993-05-01), Cook
patent: 5256775 (1993-10-01), Froehler
patent: 5332845 (1994-07-01), Urdea et al.
patent: 5495009 (1996-02-01), Matteucci et al.
Shaw et al., Gene 84(2):371-382 (abstract), 1989.
Ludwig & Eckstein, J. Org. Chem., 1989, 631-635, vol. 54.
J.E. Hay, Advances in Liver Transplantation, Jun. 1993, 337-349, vol. 22.
R.A. Stull & F.C. Szoka, Jr., Pharmaceutical Research, 1995, 465-483, vol. 12.
Horn, et al., Tetrahedron Letters, 1986, 4705-08, vol. 27.
Beuacage et al., Tetrahedron Letters, 1981, 1859-1862, vol. 22.
Moffatt, Can. J. Chem., 1964, 599-604, vol. 42.
Uhlmann et al., Tetrahedron Letters, 1986, 1023-26, vol. 27, issue 9.
Sinha et al., Nucleosides and Nucleotides, 1984, 157-171, vol. 3.
R. Iwase, Nucleic Acids Research, 1992, 1643-48, vol. 20, issue 7.
Guar, Sproat et al., Tetrahedron Letters, 1992, 3301-04, vol. 33, issue 23; Novel Solid Phase of 2'-O-methylribonucleoside 5'-triphosphates and their a-thio analogues.
Pieken et al., Science, 1991, 314-17, vol. 253.
Heidenreich et al., J. Biol. Chem., 1994, 2131-38, vol. 269.
Tuschl, Biochemistry, 1993, 11658-68, vol. 32.
Horn, DNA, 1986, 412-426, vol. 5, issue 5.
Plotch, et al., Cell, 1981, 847-858, vol. 23.
Olsen, et al., Biochemistry, 1991, 9735-41, vol. 30.
Benseler Fritz
Cole James L.
Kuo Lawrence C.
Olsen David B.
Giesser Joanne M.
Heber Sheldon O.
Merck & Co. , Inc.
Tribble Jack L.
Zitomer Stephanie W.
LandOfFree
Capped synthetic RNA, analogs, and aptamers does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Capped synthetic RNA, analogs, and aptamers, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Capped synthetic RNA, analogs, and aptamers will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1251323