Chemistry: electrical and wave energy – Processes and products – Electrostatic field or electrical discharge
Patent
1993-01-26
1994-09-13
Niebling, John
Chemistry: electrical and wave energy
Processes and products
Electrostatic field or electrical discharge
204299R, G01N 2726, G01N 27447
Patent
active
053465997
ABSTRACT:
Individual thiols present in a biological sample are separated and identified using capillary electrophoresis. S-nitrosated and oxidized derivatives of thiols are also detected using capillary electrophoresis. In addition, capillary electrophoresis may be performed on samples which have been treated with an acid, so as to achieve a pH between 2.0 and 6.8, in order to further enhance detection. Separation and detection of S-nitrosated derivatives of thiols provides a means for monitoring the extent of a disease state associated with abnormal levels of nitric oxide. Such disease states include septic shock, cardiogenic shock, hypovolemic shock, atherosclerosis, hyperhomocysteinemia, venous thrombosis, arterial thrombosis, coronary occlusion, pulmonary embolism, cerebrovascular accidents, vascular fibrosis, ectopia lentis, osteoporosis, mental retardation, skeletal deformities, pulmonary hypertension, malignancy, infections and central nervous system disorders.
REFERENCES:
patent: 5002964 (1991-03-01), Loscalzo
patent: 5025001 (1991-06-01), Loscalzo et al.
T. J. O'Shea and S. M. Lunte "Selective Detection of Free Thiols by Capillary Electrophoresis-Electrochemistry Using a Gold/Mercury Amalgam Microelectrode" Analytical Chemistry, vol. 65, No. 3 (1993) 247-250.
B. Lin Ling, W. R. G. Baeyens, & C. Dewaele "Capillary zone electrophoresis with ultraviolet and fluorescence detection for the analysis of thiols Applications to mixtures and blood" Analytica Chimica Acta vol. 255 No. 2 (1991) 283-288.
Chong, Saeho et al., "Bichemical and Pharmacological Interactions Between Nitroglycerin and Thiols", Biochem. Pharm. 42(7):1433-1439 (1991).
Grossman, Paul D. et al., "Effect of Buffer pH and Peptide Composition on the Selectivity of Peptide Separations by Capillary Zone Electrophoresis", Anal. Biochem. 173:265-270 (1988).
Holloway, C. J. et al., "The Simultaneous Analysis of Glutathione in Reduced and Oxidised Froms by Capillary Isotachophoresis", in Analytical and Preparative Isotachophoresis, Walter de Gruyter & Co., Berlin/New York, pp. 193-196 (1984).
Jocelyn, Peter C., "Spectrophotometric Assay of Thiols", Methods in Enzymology 143:44-67 (1987).
Kuhr, Werner G. et al., "Indirect Fluroescence Detection of Native Amino Acids in Capillary Zone Electrophoresis", Anal. Chem. 60:1832-1834 (1988).
Kowaluk, Elizabeth A. et al., "Spontaneous Liberation of Nitric Oxide Cannot Account for in Vitro Vascular Relaxation by S-Nitrosothiols", J. of Pharm. Exp. Ther. 255(3):1256-1264 (1990).
Park, Jeen-Woo et al., "An Unusually Stable S-Nitrosothiol from Glutathione", Arch. Pharm. Res. 2(4):257-258 (1989).
Stamler, Jonathan S. et al., "Endothelium-Derived Relaxing Factor Reacts with Protein Sulfhydryl Groups to Form Stable, Biologically Active S-Nitrosothiols", Circulation 84(4)[Suppl. 2]:II-672, Entry 2671 (Oct. 1991).
Stamler, Jonathan S. et al., "S-Nitrosylation of Proteins with Nitric Oxide: Synthesis and Characterization of Biologically Active Compounds", PNAS USA 89:444-448 (Jan. 1992).
Stamler, Jonathan S. et al., "Capillary Zone Electrophoretic Detection of Biological Thiols and Their S-Nitrosated Derivatives", Anal. Chem. 64:779-785 (1992).
Tsikas, D. et al., "Application of Capillary Isotachophoresis to the Analysis of Glutathione Conjugates", J. of Chromatography 470:191-199 (1989).
Yu, Margaret et al., "Attomole Amino Acid Determination by Capillary Zone Electrophoresis with Thermooptical Absorbance Detection", Anal. Chem. 61:37-40 (1989).
E. Jellum, A. K. Thorsrud, & E. Time "Capillary electrophoresis for diagnosis and studies of human disease, particularly metabolic disorders" Journal of Chromatography 559 (1991) 455-465.
D. Tsikas & G. Brunner "Application of capillary isotachophoresis to the analysis of glutathione conjugates" Journal of Chromatography 470 (1989) 191-199.
N. Mizobuchi, T. Ageta, K. Sasaki, & H. Kodama "Isotachophoretic analyses of cyctine homocystine, and cystathionine in urines from patients with inborn errors of metabolism" Journal of Chromatography 382 (1986) 321-325.
C. J. Holloway "Bimane conjugates of thiols: Detection and migration properties in isotachophoretic systems" Journal of Chromatography. 390 (1987) 101-110.
Y. Tanaka & W. Thormann "Capillary electrophoretic determination of S-carboxymethyl-L-systeine and its major metabolites in human urine: Feasibility investigation using on-column detection of non-derivatized solutes in capillaries with minimal electroosmosis"Electrophoresis, 11(9), (1990) 760-764.
Loscalzo Joseph
Stamler Jonathan
Niebling John
Starsiak Jr. John S.
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