Chemistry: electrical and wave energy – Processes and products – Electrostatic field or electrical discharge
Patent
1993-03-18
1994-05-24
Niebling, John
Chemistry: electrical and wave energy
Processes and products
Electrostatic field or electrical discharge
204299R, G01N 2726, G01N 27447
Patent
active
053145933
ABSTRACT:
A method for treating capillary walls exposable to protein solutes is provided that promotes reversible interactions between the protein solutes and the wall. Thus a small bore capillary tube, useful for reproducible protein electrophoretic separations, includes an interfacial phase comprising agarose that is bonded to the bore. Pretreatment of the capillary walls with silylation reagents forms an intermediate layer that provides a determinable cathodic electroosmotic flow magnitude that remains constant even if the buffer pH fluctuates between 4-7. The modified capillaries can be used repeatedly and they exhibit selectivity in protein separations.
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Hewlett--Packard Company
Niebling John
Starsiak Jr. John S.
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