Chemistry: analytical and immunological testing – Involving immune complex formed in liquid phase
Patent
1982-06-01
1984-06-12
Marantz, Sidney
Chemistry: analytical and immunological testing
Involving immune complex formed in liquid phase
422100, 422102, 422104, 436180, 436807, 436810, G01N 3354, G01N 3352
Patent
active
044542356
ABSTRACT:
An apparatus and method for transferring fluids (48) in a multi-liquid reagent protocol. Serum/plasma for a therapeutic drug assay is first obtained and mixed with a fluorogenic drug reagent (48) within a first cuvette (38). After mixing, the reaction product is accessed through a small opening (50) by tipping the cuvette (38). Fluid transfer is effected through a capillary tube (12) having an accurately calibrated bore (14), the capillary tube (12) being held between its open distal and proximal ends (30, 32) by a gripping sleeve (24). The capillary tube holder (10) is then inserted into a second cuvette where it suspends the capillary tube (12) over the liquid level (54) of a second reagent (56) in second cuvette (40). By vigorous up-and-down shaking of the second cuvette (40), the contents of the capillary tube (12) are discharged substantially uniformly throughout the liquid second reagent (56) in a short time period. Assay results are then obtained by inserting the second cuvette (40 ) and the reaction product of the second reagent within a fluorometer (60).
REFERENCES:
patent: 3525264 (1970-08-01), Nieglos
patent: 3992150 (1976-11-01), Retzer
patent: 4116638 (1978-09-01), Kenoff
patent: 4214874 (1980-07-01), White
Coe Roger N.
Marantz Sidney
Miles Laboratories Inc.
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