Capillary separation system with electric field assisted post se

Chemistry: electrical and wave energy – Apparatus – Coating – forming or etching by sputtering

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2041801, G01N 2728, G01N 2726, B01D 5702

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active

049369741

ABSTRACT:
A capillary zone electrophoresis (CZE) system provides for rapid, non-turbulent post-separation diffusional mixing of sample effluent with a fluorogenic-labelling reagent permitting sensitive detection of well-defined sample component zones. A separation capillary extends into a mixing capillary so as to define an annular gap therebetween. The effluent of the separation capillary is mixed with the labelling reagent, which is introduced through the annular gap. A power supply and opposing electrodes establish an electric field which induces electro-osmotic flow of the sample and charge-related differential electrophoretic migration to define component zones. The electric field also causes the separation capillary effluent to diverge as it issues into the mixing capillary so as to facilitate diffusional mixing with the reagent fluid flow without causing significant turbulence. Thus, fluorescence labelling is effective with minimum zone broadening. This system combines the high resolving power of CZE separation with the sensitivity of labelled fluorescence detection to attain an improved system for analyzing biological samples.

REFERENCES:
Van Vliet et al., "Post-Column Reaction Detection for Open-Tubular Liquid Chromatography Using Laser-Induced Fluorescence", Journal of Chromatography, 363 (1986) 187-198.
Scholten et al., "Fluorescence Detection of Chloroanilines in Liquid Chromatography Using Post-Column Reaction with Fluorescamine-Comparison of Reaction Types and Mixing Tees", Journal of Chromatography, 218 (1981) 3-13.
Lawrence et al., "Continuous Post-Column Ion-Pair Extraction Detection of Some Basic Organic Compounds in Normal-Phase Chromatography", Journal of Chromatography, 185 (1979) 473-481.
Tsuda & Kobayashi, "Post-Column Detection for Capillary Zone Electrophoresis", Journal of Chromatography, 456 (Dec. 1988) 375-381.
Pentoney, S. L. et al., "On-Line Connector for Microcolumns: Application to the On-Column o-Phthaldialdehyde Derivatization of Amino Acids Separated by Capillary Zone Electrophoresis", Analytical Chemistry, vol. 60, No. 23, (Dec. 1, 1988) pp. 2625-2629.
Weber, A. J. et al., "Peroxyoxalate Chemiluminescence Detection with Capillary Liquid Chromatography", Analytical Chemistry, vol. 59, No. 10 (1987) pp. 1452-1457.

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