Chemistry: electrical and wave energy – Processes and products – Electrostatic field or electrical discharge
Patent
1988-01-12
1989-09-12
Niebling, John F.
Chemistry: electrical and wave energy
Processes and products
Electrostatic field or electrical discharge
204299R, 2041801, G01N 2728, G01N 2726
Patent
active
048657077
ABSTRACT:
An improved microcapillary column for high performance electrophoresis includes a microcapillary, a hydrophilic polymer within a gel of crosslinked polyacrylamide polymerized in the tube, and preferably, a thin layer of connecting material covalently bonded to the inner surface of the microcapillary wall and to the polymeric gel. The microcapillary is prepared by first covalently bonding a suitable bifunctional reagent to the inner surface of the microcapillary wall, and then causing a mixture of the hydrophilic polymer, monomer, crosslinking agent, and polymerization catalyst to react in the bore of the microcapillary to form a hydrophilic polymer-containing gel matrix which is covalently bonded to the microcapillary wall via the bifunctional reagent. In electrophoresis, this improved gel-containing microcapillary can provide peak efficiencies in excess of 100,000 theoretical plates within separation times of less than thirty minutes, permits trace level determinations of molecular weights, and permits electrophoretic operation at fields of 1000 V/cm or higher, resulting in extremely high resolution separations.
REFERENCES:
patent: 3728145 (1973-04-01), Hjerten
patent: 3876775 (1975-04-01), Izaka et al.
patent: 4533307 (1985-08-01), Ansorge
patent: 4582868 (1986-04-01), Ogawa et al.
patent: 4600641 (1986-07-01), Ogawa et al.
patent: 4657656 (1987-04-01), Ogawa et al.
patent: 4680201 (1987-07-01), Hjerten
patent: 4690749 (1987-09-01), Van Alstein et al.
patent: 4699705 (1987-10-01), Ogawa et al.
Hjerten, S., "High Performance Electrophoresis: The Electrophoretic Counterpart of High-Performance Chromatography", Journal of Chromatography 270 (1983) 1-6.
S. Hjerten, J. Chromatography, 347, pp. 191-198 (1985).
J. W. Jorgenson & K. D. Lukacs, Clin. Chem., 27, 1553 (1981).
S. Hjerten, Chromatographic Review, 9, 122-129 (1967).
J. Calvin Giddings, Separation Science, 4(3), pp. 181-189 (1969).
Shigeru Terabe, et al., Anal. Chem., 56, pp. 111-113 (1984).
J. W. Jorgenson & K. D. Lukacs, Science, 222, pp. 266-272, (1983).
B. D. Hames & D. Rickwood, Eds., "Gel Electrophoresis of Proteins", IRL Press, Oxford and Washington, D.C., 1981.
Cohen Aharon S.
Karger Barry L.
Niebling John F.
Northeastern University
Starsiak Jr. John S.
LandOfFree
Capillary gel electrophoresis columns does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Capillary gel electrophoresis columns, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Capillary gel electrophoresis columns will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-913942