Capillary electrophoretic detection of nucleic acids

Details Capillary electrophoretic detection of nucleic acids Capillary electrophoretic detection of nucleic acids

1994-08-24

2000-04-04

Marschel, Ardin H.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

4352871, 4352872, C12Q 168

Patent

active

060459956

ABSTRACT:
Methods are provided for detection and quantitation of mixtures containing target nucleobase sequences using capillary electrophoresis. Peptide nucleic acid oligomers, complementary to the target sequences and preferably having appended detectable labels, are hybridized to the targets. Capillary electrophoresis is then performed, and the detectable label is detected and quantitated.

REFERENCES:
patent: 5490909 (1996-02-01), Wang et al.
patent: 5637464 (1997-06-01), Cohen et al.
Matthews et al. (1988) Analytical Biochemistry, vol. 169, pp. 1-25.
Chen et al., "Identification of DNA Molecules by PreColumn Hybridization Using Capillary Electrophoresis", J. of Chromatography 559: 295-305 (1991).
Goodchild, "Conjuages of Oligonucleotides and Modified Oligonucleotides: A Review of Their Synthesis and Properties", Bioconjugate Chemistry vol. 1: 165-187 (1990).
Huang et al., "Bias in Quantitative Capillary Zone Electrophoresis Caused by Electrokinetic Sample Injection", Anal. Chem. 60: 375-377 (1988).
Luckey et al., "High Speed DNA Sequencing By Capillary Electrophoresis", Nucleic Acids Research 18: 4417-4421 (1990).
McCord et al., "Capillary Electrophoresis of Polymerase Chain Reaction-Amplified DNA Using Fluorescence Detection with an Intercalating Dye", J. of Chromatography A 652: 75-82 (1993).
Rose, "Characterization of Antisense Binding Properties of Peptide Nucleic Acids by Capillary Gel Electrophoresis", Anal. Chem. 65: 3545-3549 (1993).
Janson & Ryden, Eds., Protein Purification: Principles High Resolution Methods and Applications., Ch. 17 "Capillary Electrophoretic Separations" (Thormann Firestone), VCH Publishers, New York, New York (1989).

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