Chemistry: electrical and wave energy – Processes and products – Electrostatic field or electrical discharge
Patent
1992-06-17
1994-03-22
Niebling, John
Chemistry: electrical and wave energy
Processes and products
Electrostatic field or electrical discharge
2041828, 204299R, G01N 2726, G01N 27447
Patent
active
052961168
ABSTRACT:
The present invention is directed to a technique that provides enhanced separation resolution in electrophoresis. This technique involves the use of a time-varying field strength, which may be progressively increasing or decreasing, constant or otherwise, as a function of time. The shape of the field strength with respect to time may be continuous or stepwise over time, monotonic or otherwise.
Because the mobility of different size species is a function of the applied electric field, the use of a nonuniform (time varying) electric field increases the resolving power of electrophoresis. This technique has been found to provide enhanced resolution of double-stranded DNA molecules in capillary polyacrylamide gel electrophoresis. It has been found that enhanced separation of DNA restriction fragments of 1 to more than 1000 base pairs in size has been achieved employing the technique of the present invention.
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Beckman Instruments Inc.
Harder P. R.
Liu Wen
May William H.
Niebling John
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