Capillary electrophoresis technique

Chemistry: electrical and wave energy – Processes and products – Electrostatic field or electrical discharge

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Details

204299R, 356344, 356436, G01N 2726, G01N 27447

Patent

active

053544404

ABSTRACT:
To detect bands in an electrophoresis capillary tube having a liquid separating medium, a light source and a light detector of a monitor are positioned on opposite sides of the capillary tube with the light source transmitting light through a first slit and light passing to the detector through a second slit after it has passed through the capillary tube. The first and second slits are aligned with the direction of motion of bands, have a maximum length of less than 500 micrometers and a maximum width of less than 200 micrometers. There are no vertical lengths in the capillary tube having a dimension greater than one third of its length between a sample injecting end and a detecting end of the capillary tube. A sample injector includes a vacuum chamber communicating with one end of the capillary tube to pull sample into the other end and a pressure sensor to measure the pressure in said vacuum chamber and generate a signal indicative thereof and integrate it to indicate the amount of sample pulled into the system.

REFERENCES:
patent: 4898658 (1990-02-01), Karger et al.
patent: 5207886 (1993-05-01), Lauer et al.
P. Havasi and D. Kaniansky "Simple UV Absorption Detector for Capillary Isotachophoresis" Journal of Chromatography 325 (1985) 137-149.
D. J. Rose, Jr. and J. W. Jorgenson "Characterization and Automation of Sample Introduction Methods of Capillary Zone Electrophoresis" Analytical Chemistry 1988, 60, 642-648.

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