Capillary electrophoresis technique

Details Capillary electrophoresis technique Capillary electrophoresis technique

1990-01-24

1992-12-08

Niebling, John

Chemistry: electrical and wave energy

Apparatus

Coating, forming or etching by sputtering

2041801, G01N 2726, B01D 5702

Patent

active

051695111

ABSTRACT:
To collect from an electrophoresis capillary tube containing a liquid separating medium, a sample cup has two wells containing electrolyte. The bottoms of the wells are covered with clamped-on semipermeable membrane assemblies to permit the flow of buffer ions but not the migration of separated sample. Electrical continuity for the electrophoretic migration taking place in capillary tubes is provided through the electrolyte in wells, the assembled semipermeable membranes, electrolyte buffer residing the carrier, the electrode and a conductor leading to electrical ground. Separated zones are detected, electrophoretically eluted or electrosmotically discharged from the capillary tube into the electrolyte in well where they are trapped by the semipermeable membrane in assembly.

REFERENCES:
patent: 4911807 (1990-03-01), Burd
patent: 5045174 (1991-09-01), Guzman
Donald J. Rose, Jr. & James W. Jorgenson "Characterization and Automation of Sample Introduction Methods for Capillary Zone Electrophoresis" Analytical Chemistry, 60, (1988), 642-648.
Donald J. Rose, Jr. & James W. Jorgenson,"Fraction Collector for Capillary Zone Electrophoresis" Journal of Chromatography 438 (1988) 23-34.

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