Capillary electrophoresis of glycosylated proteins

Chemistry: electrical and wave energy – Processes and products – Electrophoresis or electro-osmosis processes and electrolyte...

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204452, 510501, 510465, 510495, C25B 700

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active

055994334

ABSTRACT:
A buffer and method useful for the analysis of glycoproteins by capillary zone electrophoresis. The buffer comprises water, a sugar complexing compound, a base compound for adjusting the pH, and a zwitterionic compound. An embodiment of the buffer comprises sodium borate as the complexing compound, sodium hydroxide as the base, and 3-cyclohexylamino-1-propanesulfonic acid as the zwitterionic compound. In the method, a selected glycoprotein is subjected to capillary zone electrophoresis. The proportion or the amount of the glycoprotein is determined by quantitative analysis of the resulting electropherogram.

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Little, R. R. et al., "Interlaboratory Comparison of Glycohemoglobin Results," Clinical Chemistry, vol. 37, No. 10, pp. 1725-1729, 1991.

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