Capillary electrophoresis method with polymer tube coating

Chemistry: electrical and wave energy – Processes and products – Electrostatic field or electrical discharge

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2041807, 2041814, 2041828, 2041833, 204299R, 427230, G01N 2726

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active

051819996

ABSTRACT:
A method for achieving desired electroosmotic flow characteristics in a capillary tube having charged surface groups. An electrolyte solution containing a compound effective to stably alter the charge of the tube walls is drawn into and through the tube during while the electroosmotic flow rate in the tube is being monitored, until a desired electroosmotic flow rate is achieved. The method can be used to optimize electrophoretic separation of charged protein or nucleic acid species in a capillary tube, and to produce capillary tubes with desired charge density properties.

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