Capillary electrophoresis apparatus with improved electroosmotic

Chemistry: electrical and wave energy – Apparatus – Coating – forming or etching by sputtering

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2041801, 2041821, C25B 900

Patent

active

053207300

ABSTRACT:
An electrophoretic separation apparatus includes a capillary tube having a length, a cross section, an inlet and an outlet. A first reservoir containing a solvent and, upon injection, a solute is in fluid-flow communication with the inlet and a second reservoir containing at least a solvent is also in fluid flow communication with the outlet, the capillary thereby being filled at least with the solvent. A first power supply means applies a separation potential between the first and second reservoirs and along the length of the capillary to thereby establish an electrophoretic flow of the solute therethrough. An electrically isolated plate is juxtaposed to an external surface of the capillary tube and is connected to a second power supply. An electrostatic field is thereby applied across the cross section of the capillary tube to control the electroosmotic flow therein. The electrical isolation of the plate prevents current flow between the power supply and the plate. The plate achieves effective electroosmotic flow control even though it extends only over a small portion of the length of the capillary tube.

REFERENCES:
patent: 5092972 (1992-03-01), Ghowsi
patent: 5151164 (1992-09-01), Blanchard et al.
patent: 5180475 (1993-01-01), Young et al.
"High Performance Electrophoresis, Elimination Of Electroendosmosis and Solute Adsorption", Hjerten, Journal of Chromatography, 347 (1985), pp. 191-198.
"Analytical Chem.", Hayes et al., vol. 64, (1992) pp. 512-516.
"Capillary Electrophoresis", Ewing et al., Analytical Chemistry, 1989, vol. 61, 292A.
"Capillary Electrophoresis", Kuhr, Analytical Chemistry, 1990, vol. 62, 403R-414 R.
"Direct Control of the Electroosmosis in Capillary Zone Electrophoresis by Using an External Electric Field", Lee et al., Analytical Chemistry, 1990, vol. 62, pp. 1550-1552.

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