Capillary electrophoresis apparatus for detecting emitted fluore

Details Capillary electrophoresis apparatus for detecting emitted fluore Capillary electrophoresis apparatus for detecting emitted fluore

1995-09-28

1997-04-01

Valentine, Donald R.

Chemistry: electrical and wave energy

Apparatus

Electrophoretic or electro-osmotic apparatus

204612, 204452, 204461, G01N 2726

Patent

active

056162286

ABSTRACT:
The electrophoresis apparatus has a capillary filled with gel for use in electrophoresis. A first buffer solution container is provided for storing a buffer solution and introducing a sample labelled with a fluorescent substance into an inlet of said capillary for electrophoresis. A second buffer solution container is provided for storing a buffer solution containing a luminous solution, into which said sample is continually introduced from an outlet of said capillary after electrophoresis. Electrophoresis means is provided for subjecting said sample to electrophoresis by applying a predetermined value of voltage to said gel through which said sample is being transferred while being electrophoresed. Light receiving means is disposed underneath the outlet of said capillary to read fluorescence emitted from said sample from bottom in a direction in which said sample is approaching closer. This electrophoresis apparatus has benefits in that it can read an electrophoresis pattern of a sample of a nucleic acid or a protein obtained by subjecting the sample to electrophoresis with a high sensitivity without requiring any expensive device structure such as a laser beam source system. Thus, it is cheaper than conventional systems requiring such a laser beam system and is easier to handle.

REFERENCES:
patent: 4867559 (1989-09-01), Bach
patent: 5221454 (1993-06-01), Manian et al.
patent: 5240585 (1993-08-01), Young et al.
patent: 5246577 (1993-09-01), Fuchs et al.
Fujiwara et al., Waveguide Capillary Flow Cell for Fluoremetry, Anal. Chem., 1988, 60, 1065-1068.

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