Chemistry: electrical and wave energy – Processes and products – Electrostatic field or electrical discharge
Patent
1990-01-05
1993-08-31
Niebling, John F.
Chemistry: electrical and wave energy
Processes and products
Electrostatic field or electrical discharge
204299R, G01N 2726, B01D 5702
Patent
active
052405761
ABSTRACT:
an apparatus is disclosed for providing capillary electrophoresis, which includes an electronically controlled valve system for automatically introducing a sample into the capillary by means of a vacuum at the end of the capillary tube. This approach of sucking in the sample is extremely accurate and reproducible, and results in a minimum of band broadening. Furthermore, it enables the entire capillary electrophoresis sytem to be easily automated. An automated temperature control system is provided which enables the temperature of the capillary tube (and hence the solvent/solute system) to be controlled during electrophoresis, thereby very directly controlling pH and electrophoretic mobility. In another embodiment, the capillary is prewashed and equilibrated to achieve substantially zero charge on the capillary wall, thereby essentially eliminating electroosmotic flow and substantially improving resolution.
REFERENCES:
patent: 3664939 (1972-05-01), Luner et al.
patent: 4898658 (1990-02-01), Karger et al.
patent: 4906344 (1990-03-01), Hjerten
"High-Resolution Separations Based on Electrophoresis and Electroosmosis", Jorgenson, J. W., Lukacs, K. D., J. of Chromatography, 218 (1981) pp. 209-216.
"Capillary zone Electrophoresis", Jorgenson, J. W.,k Lukacs, K. D., Science, vol. 222, 21 Oct. 1983.
"Characterization of A Microinjector for Capillary Zone Electrophoresis", Wallingford, R. A., Ewing, A. G., Anal. Chem., 1987, 59, 681-684.
"Separation of Organic and Metal Ions by High-Voltage Capillary Electrophoresis", Tsuda, T., Nomura, K., Nakagawa, G., J. of Chromatography, 264 (1983) pp. 385-392.
"Evaluation of an Automatic Siphonic Sampler for Capillary Zone Electrophoresis", Honda, S., Iwase, S., Fujiwara, S., J. of Chromatography, 404 (1987) pp. 313-320.
"Electric Sample Splitter for Capillary Zone Electrophoresis", Deml, M., Foret, F. Bocek, P., J. of Chromatography, 320 (1985) pp. 159-165.
"Theory, Instrumentation, and Applications of Capillary Zone Electrophoresis", Lukacs, K. D., Dissertation to University of North Carolina at Chapel Hill, 1983.
"Automatic Liquid Chromatograph Injection and Sampling", Kretz, Wolfgang, and Hans-Georg Hartl, Hewlett Packard Journal, pp. 21-24, Apr. 1984.
Grossman Paul D.
Lauer Henk H.
Mead Dennis E.
Applied Biosystems Inc.
Niebling John F.
Smith Joseph H.
Starsiak, Jr John S.
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