Chemistry: electrical and wave energy – Apparatus – Coating – forming or etching by sputtering
Patent
1991-09-27
1993-05-04
Niebling, John
Chemistry: electrical and wave energy
Apparatus
Coating, forming or etching by sputtering
2041801, G01N 2726, G01N 27447, B01D 5702
Patent
active
052078868
ABSTRACT:
An apparatus is disclosed for providing capillary electrophoresis, which includes an electronically controlled valve system for automatically introducing a sample into the capillary by means of a vacuum at the end of the capillary tube. This approach of sucking in the sample is extremely accurate and reproducible, and results in a minimum of band broadening. Furthermore, it enables the entire capillary electrophoresis system to be easily automated. An automated temperature control system is provided which enables the temperature of the capillary tube (and hence the solvent/solute system) to be controlled during electrophoresis, thereby very directly controlling pH and electrophoretic mobility. In another embodiment, the capillary is prewashed and equilibrated to achieve substantially zero charge on the capillary wall, thereby essentially eliminating electroosmotic flow and substantially improving resolution.
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Grossman Paul D.
Lauer Henk H.
Mead Dennis E.
Applied Biosystems Inc.
Boys Donald R.
Grubman Ronald E.
Niebling John
Smith Joseph H.
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