Chemistry: electrical and wave energy – Apparatus – Electrophoretic or electro-osmotic apparatus
Patent
1994-10-17
1997-01-28
Niebling, John
Chemistry: electrical and wave energy
Apparatus
Electrophoretic or electro-osmotic apparatus
204453, 204601, 204602, G01N 2726, G01N 27447
Patent
active
055974686
ABSTRACT:
An apparatus is disclosed for providing capillary electrophoresis, which includes an electronically controlled valve system for automatically introducing a sample into the capillary by means of a vacuum at the end of the capillary tube. This approach of sucking in the sample is extremely accurate and reproducible, and results in a minimum of band broadening. Furthermore, it enables the entire capillary electrophoresis sytem to be easily automated. An automated temperature control system is provided which enables the temperature of the capillary tube (and hence the solvent/solute system) to be controlled during electrophoresis, thereby very directly controlling pH and electrophoretic mobility. In another embodiment, the capillary is prewashed and equilibrated to achieve substantially zero charge on the capillary wall, thereby essentially eliminating electroosmotic flow and substantially improving resolution.
REFERENCES:
patent: 4898658 (1990-02-01), Karger et al.
patent: 5240576 (1993-08-01), Lauer et al.
Richard N. Zare et al "Bias in Quantitative Capillary Zone Electrophoresis Caused by Electrokinetic Sample Injection" Analytical Chemistry, vol. 60, No. 4 (Feb. 1988) 375-372.
James W. Jorgenson and Krynn DeArman Lukas, "Zone Electrophoresis in Open-Tubular Glass Capillaries" Analytical Chemistry, vol. 52, No. 8 (Jul. 1981) 1298-1302.
James W. Jorgenson and Krynn DeArman Lukacs, "Zone Electrophoresis in Open-Tubular Glass Capillaries" Analytical Chemistry, vol. 53, No. 8 (Jul. 1981) 1298-1302.
Richard N. Zare et al "Bias in Quantitative Capillary Zone Electrophoresis Caused by Electrokinetic Sample Injection" Analytical Chemistry, vol. 60, No. 4 (Feb. 15, 1988) 375-377.
Grossman Paul D.
Lauer Henk H.
Mead Dennis E.
Grossman Paul D.
Niebling John
Smith Joseph H.
Starsiak Jr. John S.
The Perkin-Elmer Corporation
LandOfFree
Capillary electrophoresis does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Capillary electrophoresis, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Capillary electrophoresis will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-939531