Capillary column containing a dynamically cross-linked compositi

Details Capillary column containing a dynamically cross-linked compositi Capillary column containing a dynamically cross-linked compositi

1992-01-31

1993-05-25

Valentine, Donald R.

Chemistry: electrical and wave energy

Processes and products

Electrostatic field or electrical discharge

204299R, G01N 2726, B01D 5702

Patent

active

052136698

ABSTRACT:
Disclosed herein is a capillary column containing a dynamically cross-linked composition and method of use. In a particularly preferred embodiment, the dynamically cross-linked composition comprises: 1.0% polyethylene oxide; 1.0% polyethylene glycol; 1.0% ethylene glycol; 100 mM TRIS-CHES buffer; and 0.1% sodium dodecyl sulphate, where the pH of the composition is between about 8.0 and about 9.0 and the viscosity of the composition is less than about 500 centipoise. The disclosed compositions can be used for the analysis of surfactant:proteinaceous material complexes and the generation of calibration curves over an extensive range of molecular weights, using capillary electrophoretic techniques. The disclosed compositions are particularly suited for capillary electrophoretic systems having UV-based detection.

REFERENCES:
patent: 4997537 (1991-03-01), Karger
Bode, H-J. "SDA-Polyethyleneglycol Electrophoresis: A Possible Alternative to SDS-Polyacrylamide Gel Electrophoresis" 65/1:56-58 FEBS Letters (1976).
Widhalm, A. et al., "Capillary Zone Electrophoresis with a linear non-crosslinked polyacrylamide gel: Separation of proteins according to molecular mass," J. Chron. 549: 446-451 (1991).
Tsuji, K. "High-Performance Capillary Electrophoresis of Proteins. Sodium dodecyl sulphate-polyacrylamide gel-filled capillary column for the determination of recombinant biotechnology-derived proteins." J. Chron. 550: 823-830 (1991).

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