Capillary blotting pad for molecular transfer to membranes

Details Capillary blotting pad for molecular transfer to membranes Capillary blotting pad for molecular transfer to membranes

1991-04-29

1992-09-22

Niebling, John

Chemistry: electrical and wave energy

Processes and products

Electrostatic field or electrical discharge

2041801, 210679, 210681, C25B 100, B01D 5702

Patent

active

051494086

ABSTRACT:
A capillary blotting sandwich for transferring soluble macromolecules in a liquid medium from a liquid-permeable matrix to a semi-permeable receiving membrane. The blotting sandwich includes: (i) a liquid-permeable matrix layer comprising soluble macromolecules, said matrix having at least one flat surface, (ii) a semi-permeable membrane sheet layer disposed on said flat surface; (iii) an interlocked hydrophilic absorbent fiber-containing material in the form of a non-woven absorbent felt sheet or pad layer disposed on said semi-permeable membrane, wherein said felt layer facilitates capillary transport of a solution through said membrane, whereby said soluble macromolecules in said matrix layer are caused to migrate to said membrane layer.

REFERENCES:
patent: 4994166 (1991-02-01), Fernwood
Southern, "Detection of Specific Sequences Among DNA Fragments Separated by Gel Electrophoreus", J. Mol. Biol. (1975) 98, 503-517.

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