Capillary bed electrophoresis

Details Capillary bed electrophoresis Capillary bed electrophoresis

1993-06-16

1994-07-26

Niebling, John

Chemistry: electrical and wave energy

Processes and products

Electrostatic field or electrical discharge

2041828, 204299R, G01N 2726, G01N 27447

Patent

active

053324803

ABSTRACT:
A capillary bed electrophoresis device for separating and collecting large-scale electrophoretic samples is disclosed. The invention relates to an improvement in the anticonvective packing and cooling system comprising a series of capillaries through which the sample and an eluent are driven by force. The sample is separated via an electrical gradient established axially within the capillaries while a coolant may be circulated intimately around the exterior of said capillaries to remove any Joule heating. The separated components are then collected at the discharge end of the capillaries. The system may be used with either continuous or batch electrophoresis devices.

REFERENCES:
patent: 3969218 (1976-07-01), Scott
patent: 4642169 (1987-02-01), Yoshisato et al.
patent: 5045172 (1991-09-01), Guzman
Norberto A. Guzman and Maria A. Trebilcock "Capillary Electrophoresis for Analytical Separation and Semi-Preparative Collection of Monoclonal Antibodies" Analytic Chimica Acta 249 (1991) 247-255.
Y. Tarnopolsky, A New Approach to Scaling Up Electrophoresis Separation Science and Technology, 28(1-3), pp. 719-731 No month available (1993).
X. Huang, Capillary Array Electrophoresis Using Laser-Excited Confocal Fluorescence Detection Analytical Chemistry, 64 pp. 967-972 Apr. (1992).

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