Capillary array-based enzyme screening

Details Capillary array-based enzyme screening Capillary array-based enzyme screening

2005-12-06

2005-12-06

Wessendorf, T. D. (Department: 1639)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

C435S007200, C435S006120, C435S004000, C435S091500, C435S091500, C435S091500, C536S023200, C536S023100

Reexamination Certificate

active

06972183

ABSTRACT:
A process for screening an expression library to identify clones expressing enzymes having a desired activity is provided. The process involves first generating from genomic DNA samples of one or more microorganisms an expression library comprising a plurality of recombinant cell clones, and then introducing into capillaries in a capillary array a substrate and at least a subset of the clones, either individually or as a mixture. Interaction of the substrate and a clone expressing an enzyme having the desired activity produces an optically detectable signal, which can then be spatially detected to identify capillaries containing clones producing such a signal. The signal-producing clones can then be recovered from the identified capillaries.

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