Capillaries for use in a multiplexed capillary electrophoresis s

Details Capillaries for use in a multiplexed capillary electrophoresis s Capillaries for use in a multiplexed capillary electrophoresis s

1996-06-18

1997-12-09

Gorgos, Kathryn L.

Chemistry: electrical and wave energy

Apparatus

Electrophoretic or electro-osmotic apparatus

204451, 204455, 204601, G01N 2726, G01N 27447

Patent

active

056956266

ABSTRACT:
The invention provides a side-entry optical excitation geometry for use in a multiplexed capillary electrophoresis system. A charge-injection device is optically coupled to capillaries in the array such that the interior of a capillary is imaged onto only one pixel. In Sanger-type 4-label DNA sequencing reactions, nucleotide identification ("base calling") is improved by using two long-pass filters to split fluorescence emission into two emission channels. A binary poly(ethyleneoxide) matrix is used in the electrophoretic separations.

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