Cap-independent translation sequences derived from barley yellow

Details Cap-independent translation sequences derived from barley yellow Cap-independent translation sequences derived from barley yellow

1997-05-20

1999-06-08

Ketter, James

Multicellular living organisms and unmodified parts thereof and

Method of introducing a polynucleotide molecule into or...

The polynucleotide alters fat, fatty oil, ester-type wax, or...

435 691, 4352542, 435410, 435419, 536 241, A01H 104

Patent

active

059106280

ABSTRACT:
The present invention provides a method of increasing the production of a protein translated from an uncapped eukaryotic messenger ribonucleic acid (mRNA), comprising the steps of: selecting a nucleotide sequence encoding a protein to be expressed; joining nucleotides 1-168 of the barley yellow dwarf virus RNA, PAV serotype to the 5' untranslated region of said uncapped mRNA, said nucleotides 1-168 comprising the 5' untranslated region plus first 27 nucleotides of the open reading frame of the barley yellow dwarf virus RNA; linking nucleotides 4513-5677 of the barley yellow dwarf virus RNA, PAV serotype, to the 3' untranslated region of said uncapped mRNA; and expressing the protein. Also provided is a DNA molecule which comprises: (a) a promoter region which functions in plant cells to cause the production of an RNA sequence, which is operably linked to (b) a 5' untranslated region including a 5' translation enhancing segment, said 5' translation enhancing segment comprising nucleotides 1-168 of the barley yellow dwarf virus RNA, PAV serotype, said nucleotides 1-168 encompassing the 5' untranslated region plus first 27 nucleotides of the open reading frame of the barley yellow dwarf virus RNA; (c) a coding sequence, wherein said coding sequence is heterologous to said 5' untranslated region, which is operably linked to (d) a 3' untranslated region that functions in plant cells to cause the termination of transcription, said 3' untranslated region comprising nucleotides 4513-5677 of the barley yellow dwarf virus RNA.

REFERENCES:
Wang et al. The Journal of Biological Chemistry. vol. 270 (22), pp. 13446-13452, Jun. 2, 1995.

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