Canine distemper virus vaccine and method of preparation

Details Canine distemper virus vaccine and method of preparation Canine distemper virus vaccine and method of preparation

1989-12-01

1993-01-12

Brown, Johnnie R.

Drug, bio-affecting and body treating compositions

Lymphokine

424 88, 530350, 530395, 435 693, 435 701, 435 703, 435239, 4352401, 4352403, A61K 3912, C12N 500

Patent

active

051788624

ABSTRACT:
Disclosed is a vaccine for the prevention of disease caused by canine distemper virus (CDV). The vaccine is prepared from CDV immunogens derived from CDV persistently-infected cells cultured in vitro. CCL-64 mink lung cells are the preferred cell line. CDV persistently-infected cells are cultured in serum-containing growth medium, the cells transferred and maintained in serum-free medium under conditions and for a time adequate to accumulate in said serum-free medium said CDV immunogens, and the cells separated from said CDV immunogen-containing supernatant. The CDV vaccine is made by diluting said supernatent and blending with a pharmaceutically-acceptable adjuvant.

REFERENCES:
patent: 3836626 (1974-09-01), Lavender et al.
patent: 4434157 (1984-02-01), Olsen
patent: 4992272 (1991-02-01), Bass et al.

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