Canine-derived hemoglobin blood substitute, the process for prep

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Blood proteins or globulins – e.g. – proteoglycans – platelet...

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514 6, 514 2, A61K 3514

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active

060050780

ABSTRACT:
A canine-derived hemoglobin blood substitute comprising an extract of dog erythrocyte cytoplasm in a pharmaceutically acceptable carrier, the process for preparing the same and uses thereof. The process comprises utilizing membrane molecular phase transitions to induce and reverse a nonporous-porous behavior of erythrocytes, conserving all membrane and cytoskeletal material as well as all cytoplasmic enzymes including methemoglobin-reducing enzymes, yielding large quantities that do not require dialysis to remove potassium, and steps to easily prepare and use the product without extensive processing or chromatographic separation. In contrast to the prior art, the canine-derived blood substitute does not exhibit hypertension, vasoconstriction or loss of blood volume. The homologous product is intended for treatment of diseases or medical conditions of dogs requiring an increase, restoration or supplement in oxygen perfusion without hemodynamic or immunologic restriction.

REFERENCES:
patent: 5084558 (1992-01-01), Rausch et al.
Sigma on-line catalog. Product information. Product #H7130.

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