Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for...
Patent
1996-11-01
1999-03-23
Wax, Robert A.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
4352523, 43525411, 4353201, 435325, 536 232, 536 234, C12N 900, C12N 114, C12N 1500, C07H 2104
Patent
active
058858156
ABSTRACT:
The present invention relates to isolated and/or recombinant nucleic acids which encode Candida isoleucyl-tRNA synthetases, portions thereof, or fusion proteins comprising a Candida isoleucyl-tRNA synthetase or portion thereof. Also disclosed are constructs comprising the nucleic acids of the present invention, host cells comprising a recombinant nucleic acid or construct, and methods of producing a Candida isoleucyl-tRNA synthetase, portion thereof, or fusion protein comprising same. Also described are tester strains, which are cells engineered to rely on the function of a Candida isoleucyl-tRNA synthetase or functional fragment thereof encoded by an introduced cloned gene, and which can be used in a method of detecting an inhibitor of Candida isoleucyl-tRNA synthetase function.
The invention further relates to isolated and/or recombinant Candida isoleucyl-tRNA synthetases, portions thereof, or fusion proteins comprising a Candida isoleucyl-tRNA synthetase or portion thereof, methods of use of these polypeptides in an assay to identify inhibitors of Candida isoleucyl-tRNA synthetase function, and antibodies reactive with Candida isoleucyl-tRNA synthetases.
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Gallant Paul L.
Houman Fariba
Kaufmann Christoph
Kranz Janice E.
Sassanfar Mandana
Cubist Pharmaceuticals, Inc.
Hobbs Lisa J.
Wax Robert A.
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