Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Transferase other than ribonuclease
Patent
1992-07-09
1994-05-03
Robinson, Douglas W.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Transferase other than ribonuclease
435815, 424 858, C12N 910, A61K 3900
Patent
active
053087690
ABSTRACT:
A substantially purified cancer-related galactosyltransferase and a monoclonal antibody which may be used as a diagnostic reagent of cancer are disclosed. The galactosyltransferase of the present invention has a molecular weight of about 50,000 determined by SDS-polyacrylamide gel electrophoresis under reducing conditions and has a reactivity with MAb4880 under native conditions. A part of the galactosyltransferase self-associates and its reactivity with MAb4880 and the degree of self-association are promoted when it is reduced so that a part of which is detected in a fraction of a molecular weight of not less than 200,000 in gel permeation chromatography. The galactosyltransferase of the present invention can be used as a cancer marker or as a reagent for preparing a monoclonal antibody with a specificity to the galactosyltransferase, which monoclonal antibody can be used as a diagnostic of a cancer. The monoclonal antibody of the invention specifically reacts with the cancer-related galactosyltransferase of the invention.
REFERENCES:
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S. K. Chatterjee et al. "Murine Monoclonal Antibodies Against Galactosyl Transferase From The Ascites of Ovarian Cancer Patients," Chemical Abstracts, vol. 102, No. 1, 4295 x (Jan. 7, 1985).
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Uejima Takao
Uemura Morito
Yoshida Shin-ya
Konica Corporation
Robinson Douglas W.
Weber Jon P.
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