Cancer-related human galactosyltransferase GT-II

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Transferase other than ribonuclease

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435815, 424 858, C12N 910, A61K 3900

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active

053087690

ABSTRACT:
A substantially purified cancer-related galactosyltransferase and a monoclonal antibody which may be used as a diagnostic reagent of cancer are disclosed. The galactosyltransferase of the present invention has a molecular weight of about 50,000 determined by SDS-polyacrylamide gel electrophoresis under reducing conditions and has a reactivity with MAb4880 under native conditions. A part of the galactosyltransferase self-associates and its reactivity with MAb4880 and the degree of self-association are promoted when it is reduced so that a part of which is detected in a fraction of a molecular weight of not less than 200,000 in gel permeation chromatography. The galactosyltransferase of the present invention can be used as a cancer marker or as a reagent for preparing a monoclonal antibody with a specificity to the galactosyltransferase, which monoclonal antibody can be used as a diagnostic of a cancer. The monoclonal antibody of the invention specifically reacts with the cancer-related galactosyltransferase of the invention.

REFERENCES:
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patent: 4797356 (1989-01-01), Brandt et al.
D. K. Podolsky et al, Biochem. and Biophys. Res. Commun., 65 (2) 545-551 (1975), "Galactosyltransfease Activities in Human Sera: Detection of a Cancer-Associated Isoenzyme".
Uemura et al, Cancer Research, 48, 5325-5334 (1988), "Characterization and Immunoassay of Human Tumor-Associated Galactosyltransferase Isoenzyme II" 65 (2) 545-551.
The Journal of Biological Chemistry, 254, 3983-3990 (1979).
S. Nozawa, "The Usefulness And Limitation of Sugar Antigen In Ovarian Cancers. With Special Reference To a New Tumor Marker, CA54/61," Chemical Abstracts, vol. 111, No. 11, 94720v (Sep. 11, 1989).
S. K. Chatterjee et al. "Murine Monoclonal Antibodies Against Galactosyl Transferase From The Ascites of Ovarian Cancer Patients," Chemical Abstracts, vol. 102, No. 1, 4295 x (Jan. 7, 1985).
M. Uemura et al, "Antiidiotypic Antibody to Galactosyl Transferase For Immunoassay And Cancer Diagnosis." Chemical Abstracts, vol. 110, No. 21, 191089h (May 22, 1989).

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