Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2006-05-01
2010-02-16
Myers, Carla (Department: 1634)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S091100, C435S091200, C536S024300, C536S024330
Reexamination Certificate
active
07662563
ABSTRACT:
There is disclosed a cancer diagnostic method based upon DNA methylation differences at specific CpG sites. Specifically, the inventive method provides for a bisulfite treatment of DNA, followed by methylation-sensitive single nucleotide primer extension (Ms-SNuPE), for determination of strand-specific methylation status at cytosine residues.
REFERENCES:
patent: 5756668 (1998-05-01), Baylin et al.
patent: 5786146 (1998-07-01), Herman et al.
patent: 6017704 (2000-01-01), Herman et al.
patent: 6251594 (2001-06-01), Gonzalgo et al.
patent: 6811982 (2004-11-01), Gonzalgo et al.
patent: 7037650 (2006-05-01), Gonzalgo et al.
Zeschnigk et al. European Journal of Human Genetics. Mar. 1997. 5: 94-98.
Reeben et al. Biotechniques. 1994. 16(3): 416-417.
Gonzalgo et al. Nucleic Acids Research. Jun. 15, 1997. 25: 2529-2531.
Bird, “CpG-rich islands and the function of DNA methylation,” Nature, May 15, 1986, pp. 209-213, vol. 321.
Bird, “The Essentials of DNA Methylation,” Cell, Jul. 10, 1992, pp. 5-8, vol. 70.
Cedar, “DNA Methylation and Gene Activity,” Cell, Apr. 8, 1988, pp. 3-4, vol. 53.
Frommer et al., “A genomic sequencing protocol that yields a positive display of 5-methylcytosine residues in individual DNA strands,” The Proceedings of the National Academy of Sciences, Mar. 1992, pp. 1827-1831, vol. 89.
Gardiner-Garden et al., “CpG Islands in Vertebrate Genomes,” Journal of Molecular Biology, 1987, pp. 261-282, vol. 196.
Gonzalgo et al., “Identification and Characterization of Differentially Methylated Regions of Genomic DNA by Methylation-sensitive Arbitrarily Primed PCR,” Cancer Research, Feb. 15, 1997, pp. 594-599, vol. 57.
Greenwood et al., “Single Nucleotide Primer Extension: Quantitative Range, Variability, and Multiplex Analysis,” Genome Research, 1996, pp. 336-348, vol. 6.
Hatada et al., “A genomic scanning method for higher organisms using restriction sites as landmarks,” The Proceedings of the National Academy of Sciences, Nov. 1991, pp. 9523-9527, vol. 88.
Herman et al., “Methylation-specific PCR: A novel PCR assay for methylation status of CpG Islands,” The Proceedings of the National Academy of Sciences, Sep. 1996, pp. 9821-9826, vol. 93.
Jones, “DNA Methylation Errors and Cancer,” Cancer Research, Jun. 1, 1996, pp. 2463-2467, vol. 56.
Kawai et al., “Comparison of DNA Methylation Patterns among Mouse Cell Lines by Restriction Landmark Genomic Scanning,” Molecular and Cellular Biology, Nov. 1994, pp. 7421-7427, vol. 14, No. 11.
Klimasauskas et al., “Hhal Methyltransferase Flips Its Target Base Out of the DNA Helix,” Cell, Jan. 28, 1994, pp. 357-369, vol. 76, No. 2.
Kuppuswamy et al., “Single neucleotide primer extension to detect genetic diseases: Experimental application to hemophilia B (factor IX) and cystic fibrosis genes,” The Proceedings of the National Academy of Sciences, Feb. 1991, pp. 1143-1147, vol. 88.
Laird et al., “DNA methylation and cancer,” Human Molecular Genetics, 1994, pp. 1487-1495, vol. 3.
Lengauer et al., “DNA methylation and genetic instability in colorectal cancer cells,” The Proceedings of the National Academy of Sciences, Mar. 1997, pp. 2545-2550, vol. 94.
Li et al., “Targeted Mutation of the DNA Methyltransferase Gene Results in Embryonic Lethality,” Cell, Jun. 12, 1992, pp. 915-926, vol. 69.
Sadri et al., “Rapid analysis of DNA methylation using new restriction enzyme sites created by bisulfite modification,” Nucleic Acids Research, 1996, pp. 5058-5059, vol. 24, No. 24.
Schorderet et al., “Analysis of CpG suppression in methylated and nonmethylated species,” The Proceedings of the National Academy of Sciences, Feb. 1992, pp. 957-961, vol. 89.
Singer-Sam et al., “A quantitative Hpall-PCR assay to measure methylation of DNA from a small number of cells,” Nucleic Acids Research, 1990, pp. 687, vol. 18, No. 3.
Singer-Sam et al., “A Sensitive, Quantitative Assay for Measurement of Allele-specific Transcripts Differing by a Single Nucleotide,” PCR Methods and Applications, Feb. 1992, pp. 160-163, vol. 1, No. 3.
Szabó et al., Allele-specific expression and total expression levels of imprinted genes during early mouse development: implications for imprinting mechanisms, Genes & Development, 1995, pp. 3097-3108, vol. 9.
Ushijima et al., “Establishment of methylation-sensitive-representational difference analysis and isolation of hypo- and hypermethylated genomic fragments in mouse liver tumors,” The Proceedings of the National Academy of Sciences. Mar. 1997, pp. 2284-2289, vol. 94.
Xiong et al., “COBRA: a sensitive and quantitative DNA methylation assay,” Nucleic Acids Research, 1997, pp. 2532-2534, vol. 25, No. 12.
Zhou et al., “Use of a Single Sequencing Termination Reaction to Distinguish Between Cytosine and 5-Methylcytosine in Bisulfite-Modified DNA,” BioTechniques, 1997, pp. 850-854, vol. 22, No. 5.
Gonzalgo Mark L.
Jones Peter A.
Liang Gangning
Davis , Wright, Tremaine, LLP
Davison Barry L.
Myers Carla
University of Southern California
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