Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2006-05-02
2006-05-02
Myers, Carla J. (Department: 1634)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S091200, C536S024300, C536S024310, C536S024320, C536S024330, C536S025300
Reexamination Certificate
active
07037650
ABSTRACT:
There is disclosed a cancer diagnostic method based upon DNA methylation differences at specific CpG sites. Specifically, the inventive method provides for a bisulfite treatment of DNA, followed by methylation-sensitive single nucleotide primer extension (Ms-SNuPE), for determination of strand-specific methylation status at cytosine residues.
REFERENCES:
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patent: 6017704 (2000-01-01), Herman et al.
patent: 6251594 (2001-06-01), Gonzalgo et al.
Gonzalgo et al., Rapid quantitation of methylation differences at specific sites using methylation-sensitive single nucleotide primer extension (MsSNuPE).Nucleic Acids Research25(12):2529-2531, 1997.
Herman et al., Methylation-specific PCR: A novel PCR assay for methylation status of CpG islands.Proc. Natl. Acad. Sci.93:9821-9826, 1996.
Zhou et al., Use of a single sequencing termination reaction to distinguish between cytosine and 5-methylcotosine in bisulfite-modified DNA.BioTechniques22(5):850-853, 1997.
Gonzalgo Mark L.
Jones Peter A.
Liang Gangning
Davis , Wright, Tremaine, LLP
Davison Barry L.
Myers Carla J.
University of Southern California
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