Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1994-10-13
1997-11-18
Walsh, Stephen
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 405, 436503, 436811, C12Q 168
Patent
active
056886411
ABSTRACT:
A method of determining whether a test cell from a given human tissue type is (a) normal, or (b) cancerous or precancerous, by contacting the mRNA of the test cell with a nucleic acid probe which contains a nucleotide sequence at least 15 nucleotides in length which is complementary to a portion of the coding sequence of a candidate tumor suppressor gene, which gene is one that is expressed at a given control level in normal cells of that tissue type; and determining the approximate amount of hybridization of the probe to the mRNA of the test cell, an amount of hybridization one-third or less that seen with the mRNA of a normal cell of that tissue type being an indication that the test cell is cancerous or precancerous. Alternatively, an antibody specific for the candidate tumor suppressor gene product can be substituted for the nucleic acid probe as a means for determining the level of expression of the gene in the test cell. The invention also includes methods of treating a cancerous cell by increasing the level of expression of a candidate tumor suppression gene in the cell; novel candidate tumor suppressor genes; and their use in diagnosis, prognosis, and therapy.
REFERENCES:
patent: 4888278 (1989-12-01), Singer et al.
patent: 5049662 (1991-09-01), Steeg
patent: 5115096 (1992-05-01), Shoyab et al.
Ciardiello et al., PNAS 88(17):7792-6, Sep. 1, 1991.
Wildrick et al. Anticancer Res. 12(5):1271-4, Sep./Oct. 1992.
R. Sager et al., Cold Spring Harbor Symposia on Quantitative Biology, vol. LIX:537-546, 1994.
Tomasetto et al., "Specificity of Gap Junction Communication Among Human Mammary Cells and Connexin Transfectants in Culture", J. Cell Biol. 122:157-167 (1993).
Swaroop et al., "A Simple and Efficient cDNA Library Subtraction Procedure: Isolation of Human Retina-Specific cDNA Clones", Nucl. Acids Res. 19:1954 (1991).
Schweinfest et al., "Subtraction Hybridization cDNA Libraries from Colon Carcinoma and Hepatic Cancer", Genet. Annal. Techn. Appl. 7:64-70.
Cattaneo et al., Altered Ratios of Measles Virus Transcripts in Diseased Human Brains, Biology 160:523-526, 1987.
Band et al., Gene, Chromosomes & Cancer 1:48-58, 1989.
Band and Sager, Proc. Natl. Acad. Sci. USA 86:1249-1253, 1989.
Band et al., Cancer Research 50:7351-7357, 1990.
Baylin et al., Blood 70:412-417, 1987.
Calabretta et al., The Journal of Biological Chemistry 261:12628-12632, 1986.
Carr et al., Carcinogenesis 5:1583-1590, 1984.
Cowan et al., Proc. Natl. Acad. Sci. USA 83:9328-9322, 1986.
El-Deiry et al., Proc. Natl. Acad. Sci. USA 88:3470-3474, 1991.
Friedmann, Molecular Medicine Therapy for Genetic Disease, ed. by Theodore Friedmann, pp. 107-121.
Gibbs et al., Nucleic Acids Research 18:4401-4407, 1990.
Harrison et al., Proc. Natl. Acad. Sci. USA 80:6606-6610, 1983.
Jones and Buckley, Advances in Cancer Research 54:1-23, 1990.
Kligman and Hilt, TIBS 13:437-443, 1988.
Lee et al., Pro. Natl. Acad. Sci. USA 88:2825-2829, 1991.
Lee et al., Molecular and Cellular Biology 10:1982-1988, 1990.
Lersch and Fuchs, Molecular and Cellular Biology 8:486-493, 1988.
Moscow et al., Cancer Research 49:1422-1428, 1989.
Sager, Science 246:1406-1412, 1989.
Trask et al., Proc. Natl. Acad. Sci. USA 87:2319-2323, 1990.
Yaswen et al., Proc. Natl. Acad. Sci. USA 87:7360-7364, 1990.
Zhang and Nicholson, J. Cell. Biol. 109-3391-3401, 1989.
Wiedow et al., J. Biological Chemistry 265:14791-14795, 1990.
Tsunemi et al., Biochemical and Biophysical Research Communications 185:967-973, 1992.
Saheki et al., Biochemical and Biophysical Research Communications 185:240-245, 1992.
Lee et al., Proc. Natl. Acad. Sci. 89:2504-2508, 1992.
Remold-O'Donnell et al., Proc. Natl. Acad. Sci. 89:5635-5639, 1992.
Lee Sam Whan
Sager Ruth
Tomasetto Catherine Laure
Zou Zhiqiang
Dana-Farber Cancer Institute Inc.
Spector Lorraine M.
Walsh Stephen
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