Cancer-associated mar binding protein

Details Cancer-associated mar binding protein Cancer-associated mar binding protein

1995-02-13

1997-04-29

Scheiner, Toni R.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 723, 436501, 436 64, 436813, 530358, 5303877, C12Q 168, G01N 33574, G01N 33566, G01N 3348

Patent

active

056247990

ABSTRACT:
The present invention provides a substantially purified protein, p114-MBP, which has an apparent molecular mass of about 114 kDa and specifically associates with a matrix attachment region DNA sequence context (MAR). p114-MBP is characterized, in part, by having substantial MAR binding activity in malignant tumor tissue but not in a corresponding non-cancerous tissue. The invention also provides a method of detecting the presence of malignant tumor tissue in a cell sample suspected of containing malignant tumor tissue by contacting the sample with a MAR, under conditions that allow the specific association of the MAR with p114-MBP, and detecting such specific association, which indicates the presence of malignant tumor tissue in the cell sample. The present invention further provides a method of diagnosing cancer in a subject suspected of having cancer by contacting a cell sample obtained from a subject with a MAR under conditions that allow the specific association of the MAR with p114-MBP and detecting such specific association, which indicates that malignant tumor tissue was present in the sample and, therefore, is diagnostic of cancer in the subject. The invention also provides a method of determining the prognosis of a cancer patient.

REFERENCES:
Dickinson, Liliane A. et al., "A Tissue-Specific MAR/SAR DNA-Binding Protein with Unusual Binding Site Recognition." Cell 70:631-645 (1992).
Nakagomi, K et al., "A Novel DNA-Binding Motif in the Nuclear Matrix Attachment DNA-Binding Protein SATB1." Mole. Cell. Biol. 14:1852-1860 (1994).
Dickinson, Liliane A. and Kohwi-Shigematsu, Terumi "Nucleolin is a Matrix Attachment Region DNA-Binding Protein That Specifically Recognizes a Region with High Base-Unpairing Potential." Mole. Cell. Biol. 15:456-465 (1995).
Fey, Edward G. and Penman, Sheldon "Nuclear Matrix Proteins Reflect Cell Type of Origin in Cultured Human Cells." Proc. Natl. Acad. Sci. USA 85:121-125 (1988).
Khanuja, Parvinderjit S. et al., "Nuclear Matrix Proteins in Normal and Breast Cancer Cells." Cancer Res. 53:3394-3398 (1993).
Luderus, M. E. Eva et al., "Binding of Matrix Attachment Regions to Lamin B.sub.1." Cell 70:949-959 (1992).
von Kries, Jens P. et al., "Chicken MAR Binding Protein p120 is Identical to Human Heterogeneous Nuclear Ribonucleoprotein (hnRNP) U." Nucleic Acids Res. 22:1215-1220 (1994).
von Kries, Jens P. et al., "A Matrix/Scaffold Attachment Region Binding Protein: Identification, Purification, and Mode of Binding." Cell 64:123-135 (1991).

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