Agitating – Having specified feed means – Adjustable mixing ratio control
Patent
1994-07-29
1996-07-30
Cooley, Charles E.
Agitating
Having specified feed means
Adjustable mixing ratio control
3661818, 74569, 222137, 222309, B01F 1502, G05D 1102
Patent
active
055404987
ABSTRACT:
Gradient gels for electrophoresis are produced by an apparatus in which a pair of syringes are mounted on a support rack together with cams arranged for synchronous rotation. Cam followers engaged by the cams are linked to the syringe barrels, with each cam defining a distinct spiral path translated by one of the cam followers into a preselected rate or rate profile of volumetric discharge from one of the syringes as the cam rotates. With the rate or rate profile established by one cam being distinct from the rate or rate profile established by the other, a gradient or other variation in composition is obtained when the syringe discharges are combined into a common stream and directed to the gel enclosure for solidification into a gel. The gradient or variation in composition is determined by the cam profiles alone, and is independent of the rate at which the cams are rotated, and of whether or not the rotation is performed in a steady manner. The apparatus can be readily designed for manual operation with highly accurate and reproducible results.
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Bio-Rad Laboratories, Inc.
Cooley Charles E.
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