Calmodulin-based cell separation technique

Details Calmodulin-based cell separation technique Calmodulin-based cell separation technique

1997-11-14

1999-11-16

Hutzell, Paula K.

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

435378, 435 72, 436536, 436538, 436540, 436541, C12N 500, C12N 502, G01N 3353, G01N 33536

Patent

active

059856580

ABSTRACT:
The invention provides a method for separating target cells from a plurality of cells which is based on a reversible high affinity interaction between two molecules. The method comprises: forming a target cell/cell binding reagent/first molecule/second molecule/solid support complex, wherein the cell binding reagent is specific for target cells present within a plurality of cells and wherein the first molecule reversibly binds to the second molecule; removing non-target cells of the plurality of cells not attached to the solid support; and reversing the first molecule binding to the second molecule, thereby releasing the target cells as separate cells from the plurality of cells.

REFERENCES:
patent: 4812401 (1989-03-01), Tarnowski et al.
patent: 5136095 (1992-08-01), Tarnowski et al.
patent: 5182262 (1993-01-01), Leto
patent: 5304487 (1994-04-01), Wilding et al.
patent: 5370993 (1994-12-01), Tarnowski et al.
patent: 5405743 (1995-04-01), Tarnowski et al.
patent: 5518882 (1996-05-01), Lund et al.
patent: 5635358 (1997-06-01), Wilding et al.
patent: 5731425 (1998-03-01), Brizzard et al.
patent: 5773224 (1998-06-01), Grahdics et al.
O'Neil and DeGrado, TIBS 15:59-64 (1990).
Klee and Vanaman, Adv Prot Chem 35:213-321 (1982).
Billingsley et al., Proc Natl Acad Sci USA 82:7585-7589 (1985).
Hopp, T.P., et al., Bio/Technology, 6:1204-1210 (1988).
Nilsson, B., et al., EMBO J., 4:1075-1080 (1985).
Hellebust, H., et al., J. Bacteriol., 172:5030-5034 (1990).
Knappik, A., and Pluckthun, A., BioTechniques, 17:754-761 (1994).

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