Optics: measuring and testing – For optical fiber or waveguide inspection
Patent
1984-12-24
1988-08-30
Rosenberger, R. A.
Optics: measuring and testing
For optical fiber or waveguide inspection
2504612, 436 10, 436172, 356 39, 356243, G01N 2164
Patent
active
047672064
ABSTRACT:
A method of calibrating a flow cytometer is based on a set of highly uniform microbeads associated with a fluorescent dye in such a way that the microbeads have the same excitation and emission spectral properties as the samples which are to be measured. The calibration values of the microbeads are plotted against the relative fluorescence intensity peak channel for each microbead in the set. From this calibration plot, the relative fluorescence intensity peak channel of the sample is translated into equivalent soluble fluorescent dye molecules per sample particle. The calibration values of the standard microbeads are determined against solutions of the dyes. In cases where the background scatter of the bulk microbeads suspensions is too high for a direct determination against the solutions, a different set of microbeads with low background scatter is calibrated against the dye solutions and used to make an initial calibration of the flow cytometer, which in turn, is used to calibrate the uniform microbead standards. A novel method of making the microbead standards is also disclosed.
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Flow Cytometry Standards Corporation
Hultquist Steven J.
Rosenberger R. A.
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