Calibrating microarrays

Details Calibrating microarrays Calibrating microarrays

2002-10-10

2010-12-07

Yu, Melanie J (Department: 1641)

Chemistry: molecular biology and microbiology

Apparatus

C435S007100, C435S287100, C435S287200, C435S288300, C435S288400, C435S288700, C436S518000, C436S164000, C436S807000, C436S809000

Reexamination Certificate

active

07846713

ABSTRACT:
An assay for measuring the amount of a first analyte in a sample, comprises the steps of: (i) contacting the sample with a device that comprises one or more first reaction sites which comprise a first ligand having affinity for the first analyte, and a series of second reaction sites each comprising different known concentrations of an immobilized second analyte; (ii) removing any unbound first analyte; (iii) contacting the device with a second ligand that is detectably labelled and which has affinity for the first analyte, and a third ligand that is detectably labelled and which has affinity for the second analyte; (iv) removing any unbound second and third ligands; and (v) measuring the amount of second and third ligands, wherein measurement of the third ligand is used to establish a calibration curve, used to determine the amount of first analyte present in sample.

REFERENCES:
patent: 4459360 (1984-07-01), Marinkovich
patent: 5554339 (1996-09-01), Cozzette et al.
patent: 5599668 (1997-02-01), Stimpson et al.
patent: 5677196 (1997-10-01), Herron et al.
patent: 5726064 (1998-03-01), Robinson et al.
patent: 5968515 (1999-10-01), Thacker
patent: 6066448 (2000-05-01), Wohlstadter et al.
patent: 6110749 (2000-08-01), Obremski et al.
patent: 6232066 (2001-05-01), Felder et al.
patent: 6232608 (2001-05-01), Giebeler et al.
patent: 6341182 (2002-01-01), Fitzgerald et al.
patent: 6815217 (2004-11-01), Karl et al.
patent: 6916621 (2005-07-01), Shah
patent: 2002/0015958 (2002-02-01), Audeh et al.
patent: 2002/0058273 (2002-05-01), Shipwash
patent: 2002/0168692 (2002-11-01), Cass
patent: 2003/0012695 (2003-01-01), Shalon et al.
patent: 2003/0148542 (2003-08-01), Pawlak et al.
patent: 2003/0153013 (2003-08-01), Huang
patent: 2 324 866 (1998-11-01), None
patent: WO 97/46313 (1997-12-01), None
patent: WO 99/13313 (1999-03-01), None
patent: WO 01/09607 (2001-02-01), None
patent: WO 01/20330 (2001-03-01), None
patent: WO 01/92870 (2001-06-01), None

Affiliated with

Also associated with

Rating

Calibrating microarrays does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Calibrating microarrays, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Calibrating microarrays will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-4218740