Calibrating and testing immunoassays to minimize interferences

Details Calibrating and testing immunoassays to minimize interferences Calibrating and testing immunoassays to minimize interferences

1994-12-22

1996-11-05

Spiegel, Carol A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 792, 435 793, 435 794, 435 795, 435 28, 435962, 435970, 436517, 436518, 436536, 436805, 436815, G01N 3353

Patent

active

055716822

ABSTRACT:
A method for reducing interferent bias such as hemoglobin bias in immunoassays using dried slide test elements featuring peroxidase and leuco dye as the labeling mechanism. The method features the steps of:
a) calibrating an immunoassay of a target analyte by ascertaining the time at which the rate of change in density produced by a first set of the test elements for the target analyte on at least one liquid having a known amount of interferent of choice and a known amount of target analyte, crosses over or minimizes the difference compared to the rate of change in density produced by a second set of test elements substantially identical to the first set of test elements, on a liquid having the known amount of target analyte but a negligible amount of interferent of choice, and
b) conducting an assay of a patient sample of unknown amount of the target analyte by reading the rate of change in density in a third set of the test elements substantially identical to the first and second sets, caused by the sample, at a time corresponding to the cross-over time ascertained in step a).

REFERENCES:
patent: 4022577 (1977-05-01), Brooker et al.
patent: 4184923 (1990-01-01), Schubert
patent: 4835110 (1989-05-01), Seymour et al.
patent: 5171688 (1992-12-01), Hewett et al.

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