Calcium specific diaza-18-crown-6-ether chromoionophores

Chemistry: analytical and immunological testing – Metal or metal containing – Present in biological fluids

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422 56, 422 57, 422 58, 436 73, 436 79, 436164, 436166, 436169, 534670, 534751, 534770, 534797, 534816, 534831, G01N 3320

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057473455

ABSTRACT:
Diaza-18-crown-6 compounds of the formula I: ##STR1## wherein X is a phenol, naphthol or quinolinol moiety selected from those of the formulae: ##STR2## R.sub.1, R.sub.2, R.sub.3, R.sub.4 and R.sub.5 are independently selected from hydrogen, halo, cyano, nitro, (C.sub.1 -C.sub.6) alkyl, (C.sub.2 -C.sub.8) acyl, acetamido, mercapto, alkyl or arylsulfonyl, trifluoromethyl, aryl, and substituted aryl wherein the aryl moiety is selected from phenyl and naphthyl and the aryl substituent is selected from halo, cyano, nitro, (C.sub.1 -C.sub.6) alkyl, (C.sub.2 -C.sub.8) acyl, phenyl, acetamido, mercapto, alkyl or arylsulfonyl and trifluoromethyl; provided that when R.sub.1, R.sub.2, R.sub.3 and R.sub.5 are hydrogen and X is phenol, R.sub.4 is not nitro; and provided that when X is phenol and R.sub.5 is hydrogen, R.sub.1 (or R.sub.2) and R.sub.4 are not both nitro. Compounds of the above formula are useful as chromoionophores for the detection of calcium ions in solution or dry assays, but are of particular advantage when used in dry, thin-film multilayer analytical elements.

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