Calcium complexing dyes and their use in analytical compositions

Chemistry: analytical and immunological testing – Metal or metal containing – Present in biological fluids

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260396N, 422 56, 422 57, 422 58, 436 79, 436169, 534788, 534795, 534860, 534871, 540 1, 548436, 548182, 548190, 549 13, G01N 3122

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047957123

ABSTRACT:
Novel chromogenic derivatives of 1,2-bis-(o-aminoaryloxy)ethane-N,N,N',N'-tetraacetic acid are useful for the determination of calcium ions in both solution and dry assays. These compounds comprise a dye moiety which is directly conjugated to the acetic acid substituted-nitrogen atom and which enables the compounds to exhibit maximum absorbance at a wavelength generally greater than 400 nm before complexation. When the compounds are complexed with calcium ions, the absorbance shifts to a shorter wavelength.

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patent: 4594225 (1986-06-01), Argi et al.
patent: 4603209 (1986-07-01), Tsien et al.
Tsien, Biochem., 19, pp. 2396-2404 (1980).

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